摘要
作者对新生大鼠睾丸组织冷冻保存方法及保存后的睾丸组织移植进行了研究。结果表明，用二甲基亚砜作低温保护剂，４℃渗透平衡３０～４５ｍｉｎ以０．４℃／ｍｉｎ的速率降温贮存于－８０℃低温冰箱，冻存１个月和冻存３个月的睾丸组织快速复温后在体外组织培养条件下，具有良好的合成和释放睾酮的能力。将冻存１个月和３个月的睾丸组织移植到去势成龄雄性大鼠体内，移植物具有生长发育和分泌睾酮的机能。该实验结果为人类胎儿睾丸组织的冷冻保存及组织移植提供了实验基础。文章尚对二甲基亚砜对睾丸组织的损伤效应作了讨论。
The techniques for freezing neonatal testicular fragements and of viability assays for preseved testicular tissues were studied. The results showed that the testicular tissues of neonatal rats could be preserved at-80℃for three months at least with the cryopreservation techniques of slowly freezihg and rapidly thawing ,the preserved tissues still maintained both their histologic integrity and their ability to produce detectivetestosteron in vitro and in vivo,The experin1ent provided an important experiment basis for cryoperservation of human fetal testicular tisues.The injury effects of cryoprotective agent on testicular tissues were disscussed.
关键词
睾丸
保藏
生物学
移植
冷冻
testis
preservation,biological
transplatation,homologus
rats
animals,laborary
