摘要
本文对曲细精管薄片标本的制备作了较大改进，技术要点为原位灌注固定加振动切片。改进后曲细精管入水不松散，即使振动孵育，组织片亦保持完整，超微结构完好清晰，酶反应产物定位良好。表明本法适用于睾丸电镜酶组织化学研究。
eminiferous
tubules scattered after being immersed in water,This was always anunresolved problem in
research of EMenzymatic histochemistry of testis.An obviousimprovement for specimen
preparation was reported in this paper.The main techniques wereperfusion fixation in situ and
vibration sections.By using the new method,the seminiferoustubules no longer scattered in
water,the tissue pieces were well preserved even incubated invibration condition,The
ultrastructure was clear and well preserved.The reaction products ofenzymes was precisely
located.It is suggested that this method is suitable for research onenzymatic histochemical
technique of testis in electron microscopy.
出处
《湖南医科大学学报》
CSCD
1994年第6期548-550,共3页
Bulletin of Hunan Medical University
关键词
组织细胞化学
酶
电子显微镜
睾丸
histocvtochemistry
enzymes
microscopy,
electron
testis
methods
