摘要
应用荧光法、Ｃ１８反相高压液相层析和气相色谱法分别测定１７例正常人和３２例Ⅱ型糖尿病患者（１７例有并发症）红细胞内的醛糖还原酶活性、某些核普酸和糖醇浓度。结果显示：Ⅱ型糖尿病患者红细胞内醛糖还原酶的活性高于正常人；有并发症患者的酶活性又高于无并发症患者，证明高血糖能激活醛糖还原酶，激活程度与血糖浓度呈正相关。患者红细胞内ＮＡＤＰ＋、山梨醇和果糖的浓度高于正常对照；ＮＡＤＰＨ和ＮＡＤ＋浓度低于正常对照，而此５项指标在两组患者间差异无显著性。
In this paper we
determined the activity of aldose reductase,and the concentration ofsome nucloetides,sugars
and polyols in the erythrocytes of 17 controls and 32 patients withType II diabetes
mellitus(NIDDM)by the fluorometric method,C18 reverse phase HPLC andgas chromatography
respectively.Among the 32 patients,15 cases had no complications,theremainders had various
complications,The activity of aldose reductase in NIDDM patientswas significantly higher than
that of controls(P<0.05 and P<0,01 respectively)and thatof patients with complications was
significantly higher than those without complications(P<0.05).Hyperglycemia can activate
aldose reductase and positive correlation existed be-tween sugar concentration and aldose
reductase activation(r=0.90,P<0.01).The concen-trations of NADP+,sortitol and fructose in the
erythrocytes of NIDDM patients were signifi-cantly higher than those of controls(P<0.01,P<0.05
and P<0.05 respectively).The con-centrations of NADPH and NAD+in the controls were
significantly higher than those of pa-tients(P<0.01 and P<0.05),while there was no difference
between these two groups ofpatients P>0.05).The NAD+ decrease is perhaps due to
insulin-resistance of cells in NID-DM patients,therefore causing decline of glucose oxidation.
出处
《湖南医科大学学报》
CSCD
1994年第6期521-523,共3页
Bulletin of Hunan Medical University
基金
国家自然科学基金
关键词
醛糖还原酶
高血糖
核苷酸
糖尿病
并发症
aldose reductase
hyperglycemia
nucleotide
polyl
Type II dia-betes mellitus
