摘要
借助二元Ｔｉ载体系统，用农杆菌（Ａ．ｔｕｍｅｆａｃｉｅｎｓ）与子叶外植体共培养，进行黄瓜花叶病毒壳蛋白（ＣＭＶｃｐ）基因转化三个辣椒品种的工作。经过抗卡那霉素的筛选和再生培养，得到转基因株。转基因株叶的切片在含卡那霉素的培养基上表现明显的抗性。经过对后代植株的ＤＮＡ和ＲＮＡ分子杂交以及蛋白质ＥＬＩＳＡ检测表明转基因株后代染色体上带有ＣＭＶｃｐ基因，后者在叶片中表达出ＣＭＶｃｐ。经过对表达植株叶面接种ＣＭＶ病毒，表现出明显的抗ＣＭＶ病毒的性能。
Cotyledon sxplants from three pepper cultivars were co-cultured with an agrobacterium strain carrying a binary Ti vector in which a foreign CMVcp gene was inserted after the 35 spromotor After kanamycin-resistent selection and regeneration culture five transgeneplants and their offsprings were obtained. The transformation rate is 10%.Results of Southern and Northern Blots as well as ELISA tests indicated that the CMVcp gene has incorperated, transcripted and translated in transgeneplants and their offsprings.Virus inoculation tests prove the resistance of the transgeneplants and their offsprings to CMV virus.
出处
《华北农学报》
CSCD
北大核心
1994年第3期67-71,共5页
Acta Agriculturae Boreali-Sinica
