摘要
从山蚂蝗属植物分离到９株根瘤菌并鉴定为慢生型。该类菌在酵母浸出物－甘露醇（ＹＥＭ）培养基上产碱、代时６小时以上。这些根瘤菌不能利用蔗糖、苹果酸和柠檬酸，大部分菌不能利用无机氮源，所试根瘤菌耐盐能力差，生长ｐＨ范围窄（ｐＨ５－８），ｐＨ６生长最旺盛。这些菌种对所试４种抗生素敏感，其中ＭＸＤ２．ＭＸＤ３．ＭＸＤ５．ＭＸＤ７和ＭＸＤ９对所试抗生素均无抗性，所有根瘤菌在１０μ／ｍｌ结晶紫条件下仍能生长。
Nine Rhizobium strains were isolatdfrom Desmodium spp.and dentifiedas slow-growing Rhizobia.They produced alkali in yeast extract mannitol(YEM) medium and the generation time was more than six hours.These Rhizobia could not utilize inorganic mitrogen sources.AllRhizobia had poor tolerance to salt.They were capable of growth at pH values between 5 and 8and grew well at pH6.Rhizobia of Desmodium spp.were sensitive to four antibiotics and MXD2,MXD3,MXD7 and MXD9 were not resistant to any antibiotics. These Rhizobia could grow at 10μg/ml crystal violet.
出处
《华南师范大学学报（自然科学版）》
CAS
1994年第4期25-31,共7页
Journal of South China Normal University(Natural Science Edition)
关键词
山蚂蝗属
根瘤菌
生物学特性
Desmodium
Rhizobia
generation time
yeast extract mannitol medium
