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石棉纤维诱发8-羟脱氧鸟苷导致DNA损伤的体外研究 被引量:1

A Study of DNA Damage Induced by Asbestos Fibers in Vitro
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摘要 通过体外实验观察到Rhodesia温石棉,新康温石棉和UICC温石棉、青石棉、铁石棉具有诱发8-羟脱氧鸟苷(8-OH-dG)产生而损伤小牛胸腺DNA的作用,加入H_2O_2和(或)FeSO_4可明显增强石棉纤维致8-OH-dG产生的作用。以UICC青石棉纤维的作用最强,UICC铁石棉纤维次之,Rhodesia温石棉、新康温石棉和UICC温石棉纤维最弱。由此可见石棉纤维致8-OH-dG产生的作用与石棉种类和羟自由基的参与有关。作者认为8-OH-dG的产生可影响DNA中腺嘌呤与胸腺嘧啶、鸟嘌呤与胞嘧啶的碱基配对,诱发DNA点突变,与肿瘤的发生有关。 The results of our in vitro studyshowed that Rhodesia chrysotile,X.K.chrysotile,UICC chrvsotne, crocidolit and amosite could causedamage to calf thymus DNA by inducing the produc-tion of 8-hydroxy-2 ̄'-deoxyguanosine(8-OH-dG). Af-ter adding H_2O_2 and/or FeSO_4, the production of 8-OH-dG, induced by asbestos fibers was signi ficantlyincreased. The fiher of UICC crocidolite has the mostuntoward efect,that of UICC amosite comes next,and the three chrysotile fibers are the weakest ones.The effect of asbestos fibers on the production of 8-OH-dG has some relationship with hydroxy free radi-cal and the type of asbestos, It is suggested that as-bestos fibers could induce DNA point mutationthrough A·Tand G·Ctransversion by the prodac-tion of 8-OH-dG, which is related with the carcinogen-esis of asbestos.
出处 《华西医科大学学报》 CSCD 1994年第3期333-336,共4页 Journal of West China University of Medical Sciences
基金 校青年科学基金
关键词 羟脱氧鸟苷 石棉 DNA损伤 纤维 hydroxy-2 ̄'-deoxyguanosine Asbestos In vitroProject Supported by the Youth Scientiflc Research Foudation of WCUMS
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  • 1DRISCOLL T, NELSON D I, STEENLAND K, et al. The global burden of diseases due to occupational carcinogens[ J]. Am J Ind Med, 2005, 48(6) :419 -431.
  • 2联合国.温石棉暂缓列入观察清单[EB/OL].(2006-10-16)[2008-10-13].http://www.un.org/Chinese/News/fullstorynews.asp?newsID=6627.
  • 3IARC monographs on the evaluation of carcinogenic risk of chemicals to man: asbestos[J]. IARG Monogr Eval Carcinog Risk Chem Man, 1977,14:1 - 106.
  • 4RUTTNER J R, LANG A B, GUT D R, ct al. Morphological aspects of interactions between asbestos fibers and human mesothelial cell cytoskeleton[ J]. Exp Cell Biol, 1987, 55 (6) :285 - 294.
  • 5WANG N S, JAURAND M C, MAGNE L, et al. The interactions between asbestos fibers and metaphase chromosomes of rat pleural mesothelial cells in culture, A scanning and transmission electron microscopic study [J]. Am J Pathol, 1987, 126(2) :343 -349.
  • 6SESKO A, CABOT M, MOSSMAN B. Hydrolysis of inositol phospholipids precedes cellular proliferation in asbestos-stimulated tracheobronchial epithelial cells[J]. Proc Natl Acad Sci USA, 1990, 87 (19) :7385 -7389.
  • 7MARSH J P, MOSSMAN B T, DRISCOLL K E, et al. Effects of aramid, a high strength synthetic fiber, on respiratory cells in vitro[ J]. Drug Chem Toxicol, 1994, 17(2) :75 -92.
  • 8MANNING C B, SABO T, ROBLEDO R F, et al. Targeting the MEK1 cascade in lung epithelium inhibits proliferation and fibrogenesis by asbestos[J]. Am J Respir Cell Mol Biol, 2008, 38(5):618-626.
  • 9LIU J Y, BRODY A R. Increased TGF-betal in the lungs of asbestosexposed rats and mice: reduced expression in TNF-alpha receptor knockout mice[J]. J Environ Pathol Toxicol Oncol, 2001, 20(2) :97 -108.
  • 10FAUX S P, HOUGHTON C E, HUBBARD A, et al. Increased expression of epidermal growth factor receptor in rat pleural mesothelial cells correlates with careinogenicity of mineral fibres [ J ]. Carcinogenesis, 2000, 21 (12):2275-2280.

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