摘要
将6只8月龄公绵羊随机分为2组,分别经肌肉注射和滴鼻两途径,每只羊给予BVD/MDV长春184毒株第14代肾细胞培养毒澄清液20mL。结果,两种途径感染羊皆出现牛粘膜病的类似症状,并产生明显的抗体反应,中和抗体消长规律因接种途径不同而有所差异。感染羊在接种后第2周产生抗体,10～15周抗体滴度达到高峰,在1:160以上至少能持续11～17周。肌注组抗体产生较早,升高较快,持续时间短,至第27周抗体降至1:20。滴鼻组抗体升高较缓慢,持续时间较长,至第27周抗体滴度仍在1:160以上。实验还证明,BVD/MDV长春184毒株及Oregon毒株均能在MDBK、BT及牛睾丸细胞上繁殖,并有明显的细胞病变。
Six rams (eight-month old) of local breed were randomly divided into 2 groups and were infected with clarified fluid of14th passage of kidney cell culture of Changchun-184 strain ofBVD/MDV intramuscularly or intranasally. The inoculum was 20mL.All inoculated rams showed similar clinical signs of bovine virusdiarrhea-mucosal disease. Their serum neutralization antibodiesappeared from 1-2 weeks after infection and a peak titer reachedwithin 10-15 weeks. Titer levels of 1: 160 or more lasted at least11-17 weeks. Serum neutralization antibody in intramuscularly infectedrams could be detected earlier than those in intranasally infectedgroup. In the former group the titer increased slightly faster butlasted shorter as compared with later.
关键词
绵羊
粘膜病病毒
腹泻
实验感染
BVD/MDV
experimental infection
serum neutralization antibody changes
sheep
