摘要
复制失血性休克模型，采集休克前后及缺血──再灌流后颈动脉血液样本，利用放射免疫方法，测定血浆ＴＸＢ２和６—ｋｅｔｏ—ＰＧＦ１α含量变化。结果显示休克以及再灌后血浆ＴＸＢ２和６—ｋｅｔｏ—ＰＧＦ（１α）明显高于休克前水平（Ｐ＜０．０１或Ｐ＜０．０５），但再灌期与休克期相比，无显著性差异（Ｐ＞０．０５），ＴＸＢ２／６—ｋｅｔｏ—ＰＧＦ（１α）比值于休克及再灌后下降（Ｐ＜０．０５），但再灌期与休克期相比无显著性差异（Ｐ＞０．０５）。将休克前后、休克与再灌后血浆ＴＸＢ２和６—ｋｅｔｏ—ＰＧＦ１α的变化进行分析，两者无相关性（分别为ｒ＝０．１６６６和ｒ＝—０．４４０９）。本研究表明，单纯的输血输液不能明显地改善休克状态。至于休克及再灌后ＰＧＩ２变化的机理及其在休克中的作用，值得重新认识和深入探讨。
The model of hemorrhagic shock was made and blood samples in rabbits before and after shock and after ischemia-reperfusion were collected from the carotid artery. With radioimmunoassay,we measured the plasma contents of TXB2 and 6-kieto-PGF1α. The results showed that the plasma levels of TXB2 and 6-keto-PGF1α in rabbits during hemorrhagic shock and after reperfusion was significantly higher(P<0.01 or P<0.05)than those in normal.Compared with shock,the concentrations of TXB2 and 6-keto-PGF1α after ischemia-reperfusion did not change obviously(P>0.05).Compared with the normal,TXB2/6-keto-PGF1α ratio during shock and after reperfusion was reduced remarkably(P<0.05).Copared with shock,the TXB2/6-keto-PGF1α ratio following reperfusion was not signficantly different(P>0.05).The changes between TXB2 and 6-keto-PGF1α contents in rabbits before and after shock and during shock and after reperfusion showed no correlation(r=0.1666 and r=-0.4409 respectively).This study suggests that simple blood infusion can not obviously improve shock condition. The mechanism and action of alteration of PGI2 in shock merit furture investigation.
出处
《济宁医学院学报》
1994年第2期1-4,共4页
Journal of Jining Medical University
关键词
失血性
休克
缺血
再灌注
放射免疫
Hemorrhagic shock
Ischemia-reperfusion
Radioimmunoassay
TXA_2
PGI_2
