摘要
将鸡马立克氏病毒（ＭＤＶ）血清２型Ｚ＿４毒株第１０代（Ｚ＿４－１０），在对鸡马立克氏病（ＭＤ）高度易感的Ｐ系ＳＰＦ鸡胚成纤维细胞（ＣＥＦ）单层上连续传代全第３０代（Ｚ＿４－３０），Ｚ＿４毒株空斑形成速度和空斑形态均未发生显著变化。分别以Ｚ＿４－１１，Ｚ＿４－２０，Ｚ＿４－２５和Ｚ＿４－３０细胞结合毒作单价疫苗，将Ｚ＿４－１１，Ｚ＿４－２０和Ｚ＿４－３０细胞结合毒分别与Ｆ＿（Ｃ１２６）细胞结合毒组成二价疫苗，免疫Ｐ系ＳＰＦ鸡，用ＭＤＶ强毒ＧＡ株攻击，进行免疫效力试验。结果表明，Ｚ＿４毒株４个代次单价苗之间以及３个代次毒分别与Ｆ＿（Ｃ１２６）毒株组成的二价疫苗之间免疫效力无显著差异。表明Ｚ＿４毒株在ＣＥＦ单层上从第１０代连续传至第３０代，仍然保持其原有的培养特性和免疫原性。
Different passages of Z_4 strain in chicken embryo fibrablast(CEF)culture were tested to evalu-ated the stability of characteristics in culture and immunogenicity of the virus Z_4 strain of serotype 2 MDV,Z_4strain was serially passaged from passage 11 to passage 30 in CEF nomolayer cuIture originated form line P(B19/B19) SPF chickens which are highly susceptible to Marek’s disease(MD).The time interval requiredfor developing plagues, the average size and morphology of plaques, in defined period of time post-inocula-tion did not change siginificantly for Z_4 strain up to passage30.Protective efficiencies of four Z_4 monovalentvaccine, F_(C126) monovalent vaccine and bivalent vaccines respectively containing passage 11 of Z_4 and F_(C126), pas-sage 20 of Z_4 and F_(C126) or passage 30 of Z_4 and F_(C126) were compared, and the results showed that immunogenic-ity of Z_4 strain remained unchange up to passage 30.
出处
《江苏农学院学报》
CSCD
1994年第3期47-51,共5页
Jiangsu Agricultural Research
基金
农业部"七五"重点
关键词
马立克氏病
病毒
特性
免疫原
鸡病
domestic fowl
Marek’s disease virus
characteristics
immunogene
stability
