摘要
本文利用荧光染料双苯并咪唑Ｈｏｅｃｈｓｔ３３２５８对金针菇担孢子核进行了染色和观察。结果含０．６ＭＭｇＳＯ＿４·７Ｈ＿２Ｏ的Ｈｏｅｃｈｓｔ３３２５８染液对新鲜金钉菇担孢子核的染色有增效作用，并且观察到金针菇担孢子内双核占９０％以上。
The nuclei in basidospores of Flammulia Velulipes was stained and observed by usingFluorescence Dye bisbenzimide Hoechst 33258 in this paper. The staining of nuclei in basidospores offresh F,velutipes with Hoechst 33258 containing 0.6M MgSO_4.7H_2O was obviotlsly effective and bi-nuclei in basidospores of F,Velutipes was observed above 90%
出处
《江西农业学报》
1994年第2期153-155,共3页
Acta Agriculturae Jiangxi
基金
国家自然科学基金
关键词
金针茹
担孢子核
荧光染色
Flammulia velutipes
Neclei of basidospores
Fluorescence staining
Observation
