摘要
利用免疫组化ＡＢＣ法研究正常及ＣＣｌ４实验性肝纤维化大鼠肝内，Ⅰ、Ⅲ型胶原及ＰⅢＰ的分布，并对部分切片进行了图像定量分析。结果表明在正常肝脏，Ⅰ、Ⅲ型胶原在分布上一致，ＰⅢＰ在汇管区显著少于Ⅰ、Ⅲ型胶原（Ｐ＜０．００１），在肝窦周围显著多于Ⅰ、Ⅲ型胶原（Ｐ＜０．０１，Ｐ＜０．０５），提示汇管区以成熟胶原为主，在肝窦周围以新合成胶原为主。在肝纤维化时，胶原纤维分布于变性、坏死的肝细胞之间，呈“干枝挂果”样，肝窦周围的胶原随着纤维化的进展而减少。纤维隔来源于非实质细胞，肝细胞可能不参与胶原合成。
Using the immunohistochemical ABC technique, the distribution of collagen type Ⅰ, Ⅲ and P ⅢP in normal and CCl4 -induced fibrotic liver were investigated in rat. Some of the patho- logical sections were analyzed by imaging analysis system, and the collagen distribution in sinusoid and portal triads was quantified. The results revealed that the collagen type Ⅰ and Ⅲ had the same distribution pattern. In normal rat liver, the quantity of PⅢP was significantly less than:that of collagen type Ⅰ and Ⅲ in portal triads(P<0. 01) and more abundant in sinusoid(P<0. 01 , P< 0. 05 respectively). We deduced that in sinusoid,,he leading component was newly synthesized col- lagen , while the mature collagen. accounted for the majority in portal triads. In fibrotic rat liver, the collagen was distributed among the degenerated and necrotic hepatocytes, while the portal colla- gen radiated into the parenchyma, The sinusoidal collagen was gradually decreased as fibrosis pro- gressing; the mechanism of this phenomenon was unknown, The fibrotic septa was originated from the nonparenchymal cells , and the hepdtocytes might not be involved in the synthesis of collagen.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1994年第1期12-15,共4页
Medical Journal of Chinese People's Liberation Army
关键词
肝硬变
胶原
免疫组织化学
Liver cirrhosis
Experimental study of collagen
Immunohistochemistry
