摘要
研究了脱落酸（ＡＢＡ）诱导黑荆树悬浮培养细胞抗冻性发育的有关问题，其中包括ＡＢＡ施用浓度、抗冻性发育的时间进程、ＡＢＡ诱导可溶性蛋白质含量增高、环己亚胺对抗冻性发育的抑制作用等。研究结果表明，黑荆树悬浮培养细胞能够在添加ＡＢＡ的ＭＳ培养基中，在２５℃进行抗寒诱导，以１０ｍｇ／ＬＡＢＡ处理８天的悬浮培养细胞在－６℃的存活率达８３．６％，而对照存活率为零。ＡＢＡ能够诱导悬浮培养细胞可溶性蛋白质含量增高５．５倍，环己亚胺能够抑制蛋白质含量增高，同时也抑制低温驯化和ＡＢＡ诱导的抗冻性发育。
Role of abscisic acid(ABA) in the induction of freezing tolerance in Acacia mearnsii suspension-cultured cells was studied, which mainly concerned the concentration of exogenous ABA added into medium, the time course for the induction of freezing tolerance,induction of increase in soluble proteins and inhibitory effects of cycloheximide on development of freezing tolerance.The results indicated that Acacia mearnsii suspension-cultured cells could be hardened for 8 days at 25℃ by adding ABA into MS medium. Cells treated with ABA (10mg/L) attained a survival of 83.6% at-6℃, while the survival of the nonhardened(control) was 0.In comparision,the cells treated with ABA increased 5.5 fold more soluble proteins.The biosynthesis of soluble proteins was inhibited by cyclohexomide,and the development of freezing tolerance induced by cold acclimation and ABA was simultaneously prevented.
出处
《林业科学》
CAS
CSCD
北大核心
1994年第5期385-390,共6页
Scientia Silvae Sinicae
基金
国家自然科学基金
关键词
黑荆树
抗冻性
脱落酸
Acacia mearnsii
Freezing tolerance
ABA
