摘要
以牡竹属黄竹（ＤｅｎｄｒｏｃａｌａｍｕｓｍｅｍｂｒａｎｃｅｕｓＭｕｎｒｏ）的竹节及无菌苗根颈为外植体，培养于含有５ｍｇ／Ｌ２，４－Ｄ、０．２ｍｇ／ＬＫＴ和２００ｍｇ／ＬＬＨ的ＭＳ培养基上诱导愈伤组织，然后移至相同成份或不同浓度２，４－Ｄ的液体培养基中，进行悬浮培养，以建立分散性良好的悬浮细胞系。利用悬浮细胞和无菌苗叶片经酶解获大量原生质体，其产量分别约为每克鲜重２．５×１０５个和５×１０５个，活力均可达８０％。
Root crowns of plantlets and young stems with joint of Den-drocalamus
membranceus Munro were cultured on MS agar medium supplemented with 5 mg/L 2,
4-D,0.2mg/L KT and 200 mg/L LH, calli in rapid growth were formed. Then, the calli were
transferred to a liquid medium with the same medium composition or with diverse levels of 2,
4-D, the cell suspension culture which consisted maily of cell or cell aqqregates, was
established. A great amount of protoplasts were released from mesophyll of sterile seedlings
and cell suspension cultures, with protoplast yields 5×105/gFW and 2.5×105/gFW
respectively,and viability above 80%.
出处
《林业科学研究》
CSCD
北大核心
1994年第1期44-47,共4页
Forest Research
基金
浙江省自然科学基金
关键词
黄竹
细胞悬浮培养
原生质体分离
Dendrocalamus membranceus, cell
suspension culture,protoplast isolation
