摘要
本研究用反相离子对高效液相色谱法同时测定乳粉中水溶性维生素Ｖｃ，ＶＢ５，ＶＢ１、和ＶＢ２·采用ＹＧＷ－色谱柱。流支相组成ＭＥＯＨ－０．００５ＭＩＰＣ－Ｂ＿７，（３５：６５Ｖ／Ｖ，ｐＨ３．７，１％ＨＡＣ－０．３％（ＣＨ＿３ＣＨ＿２）＿３Ｎ缓冲液），流速１．０ｍＬ／ｍｉｎ，紫外检测波长２５４ｎｍ，ｔ＿Ｒ分别是Ｖ＿ｃ２．４４ｍｉｎ，ＶＢ５２．９１ｍｉｎ，ＶＢ１４．４２ｍｉｎ，ＶＢ２６．１３ｍｉｎ．样品在室温下，用盐酸进行处理。回收率为：Ｖ＿ｃ（９８．７±７．３４）％，，ＶＢ５（９９·１±５．４５）％，ＶＢ１（９２．４±８．２７）％及和ＶＢ２（９０．９±２．５４）％。
Water-soluble vitarnins V_c,VB5,VB1 and VB2 in milk powder were determined by re-versed-phase ion-pair HPLC,YGW-C_18(4.6 × 250 mm l0μm)was used as column. MobilePhase consisted of MeOH-0.005M IPC-B_7(35: 65 v/v,pH3.7(1%HAC-0.3%(CH_3CH_2)3Nbuffer, flow rate 1.0 ml/min. UV absorotion was measured at 254 nm. The retentiontime was V_c 2.44’,VB5 2.91’,VB1 4. 42’,and VB2 6.13’,respectively. Samples were preparedwith HCL at room temperature. The sample recovery was as follow ; V_c(98.7±7.34)%。VB5(99.1±5.45)%,VB1(92.4±8.27)%and VB2 (90.9±2.54)%
出处
《南昌大学学报（理科版）》
CAS
1994年第2期129-134,共6页
Journal of Nanchang University(Natural Science)
