摘要
在完整细胞与3H-R1881特异结合的基础上,用STM-TritonX-100缓冲液分离粗制核,建立了简便而快速地检测核雄激素特异结合的方法。对正常男性包皮成纤维细胞核与3H-R1881特异结合的Scatchard及单点分析表明,该细胞核与3H-R1881的特异结合具有高亲和力、低容量的特征,当细胞与近饱和浓度的3H-R1881温育50min后,47.16±7.99%(n=20)的特异结合被发现存在于核内,而一睾丸女性化(tfm)综合征患者的核特异结合量明显低于该值。我们还显示,正常男性包皮成纤维细胞与3H—R1881在42℃温育时的特异结合量比在37℃温育时减少,但其量低于40%。当减少量超过40%时,表明存在热不稳定型的雄激素受体。
By using a simple method, the presence of saturable and high affinity specific binding sits of 3H-R1881 in isolated crude nuclei of cultured genital skin fibroblsts were revealed.When intact foreskin fibroblasts from 20 normal men were incubated at 37 C with saturating concentration (2. 4 nmol/L ) of 3H-R1881, 47. 16±7. 99% of the specific binding was recovered in crude nuclei. Temperature stability of androgen receptors (AR) in fibroblasts was also tested.Specific binding of 3H- R1881 in these cells from normal men decreased by less than 40% when the incubating temperature is raised to 42℃. A greater than 40% decrease of specific binding at 42℃ compared to the value at 37℃ was considered evidence for thermolability.
出处
《男性学杂志》
CSCD
1994年第3期133-137,共5页