摘要
本研究探讨了在成熟培养液和发育培养液内添加ＥＧＦ和ＴＧＦ－α对牛体外受精卵发育的影响，在成熟培养液内分别添加ＥＧＦ和ＴＧＦ－α，经体外受精处理后卵裂率分别为７８．３％和８２．６％，显著高于对照组６０．２％的卵裂率；三个处理组囊胚发育率分别为１９．６％、２４．５％和１８．７％，三者间无显著差异．在发育培养液内分别添加ＥＧＦ和ＴＧＦ－α，其囊胚发育率分别为３０．０％和２３．４％，与对照组２１．０％的囊胚发育率相比差异不显著．在成熟培养液内添加ＴＧＦ－α，在发育培养液内添加ＥＧＦ，经这两种生长因子分别作用后，体外受精卵翼胚发育率为２１．０％，与单独使用其中一种生长因子相比囊胚发育率无明显提高．
his research observed the effects of EGF and TGF-αsupplemented to maturation anddevelopment culture medium on the development of bovine IVF ova. After adding EGF and TGF-αto maturation medium, the cleavage rates of IVF ova were 78.3%and 82.6%,respectively,which were much higher than that of the control treatment(69.2%).The development rates ofblastocyst of the three treatment were 19.6%,24.5%and 18.7%respectively,there were no sig-nificant difference among them. After adding EGF and TGF-αto development medium,the ratesof blastcyst were 30.0%and 23.4%, respectively ,and the blastocyst rate of control treatmentwas 21.0%,there were also no significant difference among them. After adding TGF-αto matura-tion medium and EGF to development medium ,the rate of blstocyst was 21.1%,it was showedthat the effect of interaction of the two growth factors has no significant difference with that ofsingle growth factor which was added to.
出处
《内蒙古大学学报（自然科学版）》
CAS
CSCD
1994年第2期215-218,共4页
Journal of Inner Mongolia University：Natural Science Edition
关键词
生长因子
体外受精
体外发育
牛
growth factor in vitro maturation in vitro fertilization in vitro development
