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扁蓿豆体细胞胚的诱导和植株再生 被引量:1

INDUCTION OF SOMATIC EMBRYOS AND PLANTREGENERATION OF MELISSITUS RUTHENICA L.
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摘要 扁蓿豆实生苗的根、下胚轴、子叶、叶片和叶柄外植体,在含2,4—D2—0.25mgL-1与KT0.25-2mgL-1及2,4—D0.5mgL-1与ZT0.5mgL-1或BAP0.5mgL-1与NAA0.05mgL-1的MS琼脂培养基上均可产生愈伤组织.愈伤组织在含2,4—D0.5—0.1mgL-1与KT0.5—0.1mgL-1或BAP0.25+NAA0.05mgL-1的MS培养基上可诱导分化出体细胞胚.体细胞胚在无激素的培养基上发育成完整植株.用海藻酸钠包襄体细胞胚制成人工种子,其发芽率和植株转换率分别为95%和53%. Explants of root,hypocotyl,cotyledon,leaf and petiole from seedlings of Melissitusruthenica L.could produce callus tissues when they were cultured on MS agar mediumcontaining 2,4-D 2-0.25mg L-l and KT 0.25-2mg L-1,or 2,4-D 0.5mg L-1 and ZT0.5mg L-1,or BAP 0.5mg L-1 and NAA 0.05mg L-1.Callus tissues Could be induced todifferentiate somatic embryos when they were transfered to MS medium containing 2,4-D0.5-0.1ing L-1 and KT 0.5-0.1mg L-1,or BAP 0.25mg L-1 and NAA 0.05mg L-1.Somatic embryos could develop into whole plants on hormone free MS medium.Somaticembryos were encapsulated by 1.5% alginate to prepare artificial seeds,of whichgermination rate and plant conversion rate were 95% and 53%, respectively.
机构地区 北京大学生物系
出处 《热带亚热带植物学报》 CAS CSCD 1994年第4期73-78,共6页 Journal of Tropical and Subtropical Botany
关键词 扁宿豆 体细胞胚 植株再生 豆科 组织培养 Melissitus ruthenica L.,Callus tissue, Somatic embryo,Artificial seed, Plantregeneration
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