摘要
邻苯二甲醛衍生高效液相色谱法快速测定血小板中组胺含量谢大年，陈亚立，郭兆贵（湖南医科大学临床药理国家培训中心长沙４１００７８）１前言组胺是一种重要的生物活性物质，在生理和药理等研究领域测定组织或体液中组胺的含量是十分必要的。国内外已报道了用生物法￣［...
n HPLC method for the determination of histamine(HA)in platelet.based on pre-column derivatiza-tion with o-phthalaldehvde(OPA ).is described.The HA was allowed to react with OPA at -10℃ for30min.HPLC was performed on a reversed-phase column with isocratic elution using sodium acetate buffer inmethanol as eluent system.A fluorescence detection system was used;excitation was set at 350.0nm andemission was read at 445.5nm,A good linear relationship was found in the range of 3.75×10￣(-3)～10. 5nmol.The detection limit,coefficient of variation and recovery were 3.75pmol.3.7% and 90.5%. respectively.
出处
《色谱》
CAS
CSCD
北大核心
1994年第1期65-66,共2页
Chinese Journal of Chromatography
关键词
血细胞
组胺
邻苯二甲醛
液相色谱
o-phthalaldehyde,high performance liquid chromatography,histamine,rapid analysis,pre-column derivatization,platelet
