摘要
高效液相色谱法测定大鼠肺及脑组织中细胞膜磷脂周建忠,邢成,王鲁明(军事医学科学院仪器测试中心北京100850)1前言细胞与周围环境的联系和反应都必须通过细胞膜来完成 ̄[1].磷脂是细胞膜的重要组成,它对细胞的功能起着重要作用 ̄[2]。目前测定磷脂的方...
A successful method for separation and quantitative determination of phosphatidylinositol(PI),phos-phatidylserine(PS),phosphatidylethanolanune(PE)and phosphatidylcholine(PC) in membrane of rat lungand brain tissues by HPLC has been developed.Under the selected conditions ,the averagerecoveries of themwere 84.14~88.43%, The contents of PI, PS, PE and PC(mean±SD)per gram of tissues in the mem-brane of normal rat lung were 201.1±20.8μg,350.6±20.0μg,162.0±11. 6μg and 24.66±2.04mg respec-tively. In the membrane of normal rat brain,the contents of them were 425.2 ± 30.2μg,2862 ± 326μg,878.4±55.7μg and 32. 83±3.73mg per gram of tissues respectively.
出处
《色谱》
CAS
CSCD
北大核心
1994年第6期441-442,共2页
Chinese Journal of Chromatography
关键词
高效液相色谱
大鼠
肺
脑组织
细胞膜磷酯
high performance liquid chromatography,phosphatidylinositol,phosphatidylserine,phosphatidylethanolamine,phosphatidylcholine,membrane of rat lung,membrane of rat brain tissue