摘要
用半固体－液体两步法克隆的人ＬＡＫ细胞不仅表现对ＮＫ敏感的Ｋ５６２细胞和ＮＫ抵抗的ＨＬ－６０细胞强烈的细胞毒性，在二次杀瘤过程中表现同样高的活性，而且ＬＡＫ细胞培养上清对白血病细胞系ＨＬ－６０和新鲜白血病细胞具有增殖抑制和分化诱导作用。然而不同杀伤活性的ＬＡＫ细胞培养上清对新鲜白血病细胞增殖的影响不同。结果表明ＬＡＫ细胞在直接杀伤白血病细胞的同时，还通过分泌一些活性因子间接影响白血病细胞的增殖和促进其分化成熟，反映了ＬＡＫ细胞抗白血病作用的多向性。
Human LAK cells were cloned by two-step method of semisolid-liquid culture with IL-2 stimulation. The LAK cells conditioned medium (LAK-CM) was obtained by centrifuging suspension from single cloning LAK cells cultured at 3-days. Cloned LAK cells showed cytotoxic to a variety of leukemic cell lines including NK-sensitive K562,and NK-resistand HL-60 cells measured by a 4-hour 51Cr release assay and still maintained high cytotoxicity in secondary killing of leukemia cells. It was found that LAK-CM possessed two different kinds of activities i. e. suppression and induction of differentiation to leukemia cell line HL-60 and fresh leukemia cells. however LAK-CM from LAK cell with different cytotoxicity have varied effects on fresh leukemia cells.Taken together these results it is suggested that LAK cells might secret some unknown activities or factors which indirectly inhibit the proliferation and/or induce the differentiation of leukemia cell.
出处
《上海免疫学杂志》
CSCD
北大核心
1994年第6期328-331,共4页
Shanghai Journal of Immunology
关键词
克隆化
自然杀伤细胞
白血病细胞
细胞毒
LAK cell clone LAK-CM human leudemia cells cytotoxicity induction of differentiation
