摘要
本文将ＨＲＰ注入一侧终纹床核，又将Ｆｏｒｍａｌｉｎ导入胃内造成损伤，通过ＨＲＰ呈色、抗ＦＯＳ、抗５－ＨＴ免疫组化染色，在中脑导水管周围灰质发现七种不同标记或免疫反应阳性细胞，即ＨＲＰ逆行标记、５－ＨＴ样阳性和ＦＯＳ样阳性细胞，ＨＲＰ／５－ＨＴ、５－ＨＴ／ＦＯＳ和ＨＲＰ／ＦＯＳ双重标记或免疫反应阳性细胞以及ＨＲＰ／５－ＨＴ／ＦＯＳ三重标记或免疫反应阳性细胞．ＨＲＰ／５－ＨＴ／ＦＯＳ细胞数量很少，出现于中脑导水管周围灰质腹外侧区。
Hor.seradish peroxidase(HRP) was stereotaxically injected into the bed nucleus of stna terminalis(BST),and visceral noxious stimulation was given by injecting formalin into the stomach. The sections were subjected to HRP reaction combined with imrnunohistochemical reactions for FOS protein (ABC method) and 5-HT-like immunoreactive(LI) cells (PAP method).Seven kinds of cells (HRP retrograde labelled cells, 5-HT and FOS-LI cells; HRP/5HT-,5-HT/FOS- and HRP/FOS-LI double labelled cells; HRP/5HT/FOS-LI triple labelled cells) were observed. Among single labelled cells, the number of FOS-LI positive ones was most numerous, they were mainly lorated in the dorsal parts of ventrolateral subdivisions at the middle and caudal levels of the periaqueductal gray(PAG). HRP single labelled and 5-HT-LI cells were mederate in number and were mainly observed in the ventrolateral subdivisions of the PAG.Among double labelled neurons, HRP/5-HT-LI double labelled neurons were most numerous. SHT/FOS-LI double labelled neurons were less in number, whereas only a few HRP/FOM-LI double labelled neurrons were found. A few HRP/5HT/FOS-LI triple labelled cells were distributed in the ventrolateral subdivisions of the PAG. The present study demonstrated that serotoninergic neurons in the PAG projected to the BST, and some of these neurons expressed FOS protein following visceral noxious stimulation.
出处
《神经解剖学杂志》
CSCD
北大核心
1994年第4期335-340,共6页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金
关键词
中脑导水管
周围灰质
终纹床核
血清素
神经元
periaqueductal gray
bed nucleus of the wtria terminalis
HRP
5-HT
FOS
triple labelling methed
noxious Stimulation
rat
