摘要
采用新型Ｃａ￣（２＋）荧光指示剂Ｆｕｒａ－２建立双波长荧光法测定分离的大鼠神经细胞内游离钙浓度（［Ｃａ￣（２＋）］＿ｉ）．结果显示，在静息状态下，其［Ｃａ￣（２＋）］＿ｉ为１０９±１２ｎｍｏｌ／Ｌ．３０ｍｍｏｌ／ＬＫＣｌ可显著增加［Ｃａ￣（２＋）］＿ｉ，并且ＫＣｌ的这种效应呈一定的剂量依赖关系，提示该法灵敏、可靠．
he intracellular free Ca￣(2+) concentration([Ca￣(2+)]) was measured in isolated neurocytesprepared from Sprague-Dawley rats with Fu-ra-2 double-wavelength fluoremetry. The re-sults showed that the [Ca￣(2+)]i, of the restingneurocytes in Ca￣(2+)-containing solution was 109+12nmol/L(-x±s, n=9).KCl (30mmol/L)markedly evoked[Ca￣(2+)]of the neurocytes(P<0. 01, n=9),and the rises of [Ca￣(2+)]i byKCl is a concentration-dependent response.These results suggest that the adoption of Fu-ra-2 double-wavelength fluoremetry in dissoci-ated rat neurocytes in a useful and relativelyeasily applicable technique for monitoring in-tracellular Ca￣(2+) changes.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第3期275-277,共3页
Progress In Biochemistry and Biophysics
基金
江苏省自然科学基金
关键词
荧光指示剂
神经细胞
游离钙
Fura-2,intracellular free calci-um. neurocytes,rat
