摘要
用较少的抗原量免疫绵羊，采用活体采血法，定期加强免疫，定期补铁，定期采血，获得抗人促甲状腺激素抗血清，采用放免分析方法鉴定抗血清，结果为：抗血清滴定为２８－２０５万，与促卵泡激素，促绒毛膜生长激素，促黄体生成素交叉都小于０．０２‰，亲和常数Ｋ大于１０＾１０Ｌ／ｍｏｌ。
pecific high titre antisera to TSH were raisedin two sheeps injected with 100μg (booster in-jection. 50μg) highly purified TSH preparationby the multi-site intradermal immunizationtechnique. Blood were bled at two week inter-vals by cardiac puncture without killing the an-imals and solution of anti-anemia drug was giv-en to sheeps after each leting blood. The antis-era were monitored by TSH RIA Kit. Titreswere range from 28 × 10￣4 to 205× 10￣4 and nocross-reaction occured between TSH antiseraand human LH, FSH, HCG and all antiserahave the avidity more than 10￣(10)L/mol.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第4期366-368,共3页
Progress In Biochemistry and Biophysics
