摘要
用对苯二酚和甲醛在酸催化下制得新一类凝胶，此凝胶价廉，易于制备，多孔、无毒、亲水性强；能选择性吸附多糖，而用于多糖与低聚糖及单糖的分离；可作为载体对多种酶及蛋白质给予固定，与蛋白质的最大结合量为５５８ｍｇ／ｇ，固定糖化酶时的活力回收达８４％；此固化酶对淀粉的转化率达９３％，当加入一定量的间苯二酚，可得改性的ＰＦ凝胶，其性能又有提高，可见ＰＦ凝胶为新型的酶的固定化优良载体。
F gel-type resin has been synthesised by poly-condensation of hydroquinone with formalde-hyde in the presence of acidic solution.Thegel-type resin is cheap and easy to preduce,non-toxic.porous.hydrophilic, extremely sta-ble.It is a effective carrier for the simple andrapid immobilization of various enzymes andproteins.The amounts of bound protein(BSA) and glycoamylase in one gramme of thedry carrier were 558mg and 330mg respective-ly,the activity recovery of 84/00 for immobi-lized glycoamylase was obtained. The cover-sion(%)for starch into glycoase, using immo-bilized glycoamylase is as high as 93%.A newkind of modified PF gel-type resins were syn-thesised by copolymerization of hydroquinoneand some resorcin with formaldehyde.ThusPF and modified PF gel-type resins are superi-or carriers for immobilization of enzyme.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
1994年第6期523-527,共5页
Progress In Biochemistry and Biophysics
基金
河北省科委资助课题
关键词
酚醛树脂
对苯二酚
甲醛树脂
固定化
凝胶
载体
phenolic-formaldehyde resin, hy-droquinone-formaldehyde resin.immobiliza-tion, glycoamylase.bovine serum albumin(BSA)
