摘要
从金顶侧耳子实体中分离纯化一半乳甘露聚糖ＰＣ－３，其分子量约为６７ｋＤ，一级结构为α（１→６）糖苷键相连的Ｇａｌ构成分子的主链，部份残基Ｃ_２上带有分支，分支结构为α（１－２）Ｍａｎ－α（１－２）Ｍａｎ。按高碘酸氧化、部份酸水解，甲基化、硫酸化程序对ＰＣ－３的结构进行化学修饰；并分别将ＰＣ－３及其衍生物与柯萨奇病毒Ｂ_５进行体外实验，结果表明，ＰＣ－３经硫酸化后，显著地提高了抗病毒活性；而高碘酸氧化，甲基化，部份酸水解产物则降低了抗ＣＢ_５的活性。
A D-Galacto-D-Mannan isolated and purified from fruit bodies of Pleurotus Citrinopileatus. The molecular weight was abount 67kD. The main chain of the molecules composed of α-D (l→6) linked Gal, side chain was α(1─2) Man a (1─2)Man. Chemical modification of PC-3 by periodate oxidation, partial acid hydrolysis, methylation, sulfation, PC-3 and its derivation were tested for anti-virus activities against Coxsackis B5. Results showed that sulfated PC-3 may increase the anti-virus (CB5) activity, other derivatives decrease the antivirus activity.
关键词
金顶侧耳
多糖
抗病毒药
药理
Pleurotus Citrinopileatus polysacchrids
Coxsackie B_5
