摘要
研究背景乳精吸收不良（LM）和乳糖不耐症（LI）确定诊断最可靠的方法是活检直接测定乳糖酶活性，然而其侵入性至今仍难作为常规手段应用。因此寻一简便可信又安全经济的间接法十分必要。研究方法受试者为4岁～7岁的健康儿童，参考Arola的乙醇乳糖耐量试验（LTTE）血、尿半乳糖测定法（血、尿法），修改其中半乳糖氧化酶法为邻苯胺法测定血半乳精（B－gal）和尿半乳糖（U－gal）。研究结果重复性和回收率均符合要求，静脉血半乳精含量为0．325±0．076mmol／L毛细血管血为0．304±0．078mmol／L，无显著差异（P＞0．1）；尿法与血法相比，敏感性为94．1％，特异性为91．6％。结论邻甲苯胺法测定B－gal和U－gal可作为LD的间接诊断法之一，静脉血和毛细血管血测定结果无显著差异。毛细血管血法易被小儿及家长接受，且尿法更为简便。
Background The reliable diagnostic method for lactose malabsorption (LM) and Lactose intolerance (LI) is the direct measurement of lactase activity with biopsy, but the traumatic nature is too large to use in routine way. There for, to search for a handy, reliable, safety and economical indirect diagnostic method is very necessary.Methods Subjects are health children aged from 4 to 7 years old. The blood and Urine galectose (B-gal and U-gal) were determined by consulting Arola blood and urine galactose measurement after lactose tolerance test with ethanol (LTTE), and use o-toluidine instead of galactose oxidase in this test.Results Repetitive nature and rate of recovery accord with the requirernent. The content of galactose is 0.325±0.076mmol/L, in venous blood and 0.304±0.078mmol/L in capillary blood (p>0.1), there is not significant difference between them. Comparing with blood method, the sensitivity and specificity of urine method are 94 .1 % and 91. 6 % respectively.Conclusions To determine the B-gal and U-gal, O-toluidine method can be regarded as an indirect diagnostic method of LD.
出处
《实用儿科临床杂志》
CAS
CSCD
1994年第1期3-5,共3页
Journal of Applied Clinical Pediatrics
关键词
乳糖酶缺乏
乙醇乳糖耐量
试验
lactase deficiency
Lactose tolerance test with ethanol
blood galactose
urine galactose
