摘要
为了探讨HBV标志的表达与肝细胞损伤的关系,本文应用 PAP 法和 ELISA 法检测了70例 CH-B 患者肝组织 HBsAg、HBcAg 和五项血清 HBV 标志。结果发现 HBsAg 在肝内有4种表达型式,HBcAg 有3种表达型式。HBsAg 和 HBcAg 在不分表达型式的情况下,CPH 的检出率高于 CAH,但按不同表达型式进行比较,则膜型 HBsAg 和浆膜型 HBcAg 的检出率反以CAH 为高,提示这二型抗原的表达与肝细胞损伤程度具有一致性。表示病毒复制的血清 HBsAg+HBeAg+抗-HBc 与 HBsAg+抗-HBc 联合检出率在 CAH 组高于 CPH 组,HBeAg 的检出率随着 SGPT 值的增高而增高。表明作为病毒复制指标的血清 HBV 标志也能反映肝细胞损伤情况。本文对靶抗原和肝组织与血清 HBV 标志表达的关系进行了讨论。
To understand the relationship between the expression of HBV-markers andinjury of hepatocytes,we have examined HBsAg,HBcAg in liver tissue with PAPmethod and HBV-markers in serum with ELISA method of 70 patients with CH-B.The results showed that the expression patterns of HBsAg could be divided into 4types,those of HBcAg into 3 types.The positive percentages of HBsAg and HBcAgin CPH were higher than in CAH under which the expression patterns were notconsidered.When considering the expression patterns,positive percentages of mem-brane type of HBsAg and cytoplasmic membrane type of HBcAg in CAH were higherthan in CPH.It was suggested that the expressions of HBV-Ag of these types wereconsistent with the degree of hepatocytic injury.The combination positive percen-tages of HBsAg+HBeAg+Anti-HBc,and HBsAg+Anti-HBc in serum in CAH,which indicated HBV replication,were higher than in CPH.The positive percen-tage of HBeAg increased along with the increase of SGPT.It revealed that HBV-markers in serum which indicated HBV replication could also reflect the conditionof hepatocyte injury.Target antigen and relation between the expression of HBV-markers in liver tissue and in serum were discussed.
出处
《天津医药》
CAS
1989年第2期79-82,共4页
Tianjin Medical Journal
