摘要
目的采用免疫亲合柱净化,结合柱后衍生化的高效液相色谱荧光检测器检测常用中药材中的黄曲霉毒素B1,B2,G1,G2。方法样品经甲醇水(7∶3)溶液超声提取后,通过免疫亲合柱净化洗脱,再经过溴化溴化吡啶柱后衍生,高效液相色谱分离定量。结果B2和G2的最低检出限为006μg·kg-1,B1和G1的最低检出限为020μg·kg-1。回收率实验添加两个水平的标样,回收率904%～997%,RSD3%～12%。结论采用此方法检测常用中药材中的黄曲霉毒素无干扰性杂峰,结果准确可靠。
Objective:To develop a new and accurate method to quantify aflatoxins in medicinal herbs. Method: This method consists of sample extraction by using MeOH-H_2O (7∶3), followed by clean-up with an immunoaffinity column, and finally HPLC determination with fluorescence detection. Aflatoxins B1 and G1 are determined as their bromine derivatives, produced in an on-line post-column derivatization system. Result:The overall average recoveries for different medicinal herbs spiked at two levels of standards were from 90.4% to 99.7%. The detection limit was 0.06 μg·kg^-1 for aflatoxins G_2 and B_2, and 0.20 μg·kg^-1 for aflatoxins G_1 and B_1. Conclusion: The use of immunoaffinity column provides excellent cleanup of interfering substances. The method has been applied successfully to analyze 96 natural drugs. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2005年第3期182-184,共3页
China Journal of Chinese Materia Medica
基金
国家"十.五"重大科技专项(2001BA701A57)
外经贸技三函(2001-18)
