摘要
目的 建立HPLC法测定醋酸泼尼松片的含量。方法 采用Diamond C18色谱柱(5μm,4.6×150 mm)为分离柱;流动 相:甲醇-水(70:30),检测波长:238 nm,以峰面积计算 结果 线性范围:0.1-0.3 mg／mL,r=0.9992。平均加样回收率为 100.7±0.9％。结论 本法简便,快捷,灵敏,可作为醋酸泼尼松片质量控制的有效方法。
Objective To establish an HPLC method for the determination of prednisone acetate tablets. Method Analytical column was Diamond C18(5 um,4.6×150 mm),the mobile phase was composed of methanol-water(70:30).The Detection wavelength was 238nm and the peak area was calculated. Result The calibration curve was linear within the concentration range of 0.1-0.3 mg/ mL. The recovery of prednisone acetate was 100.7±0. 9% .Conclusion The method is simple,rapid and accurate.
出处
《广东药学》
2005年第1期6-7,共2页
Guangdong Pharmaceutical Journal
