摘要
模拟稻秸原位还田条件,分别在水稻土和红壤中添加水稻秸秆培养70d ,第0、5、2 5、4 5、70天采集土样。采用非机械破壁法直接提取水稻土和红壤细菌总DNA ,水稻土细菌总DNA经过二次纯化;红壤细菌总DNA经过一次纯化后,PCR扩增其16SrDNAV3可变区,均可获得清晰的目的条带,对扩增产物进行DGGE分析,结果显示:水稻土和红壤样品的DGGE条带增加,说明稻秸能够增加土壤细菌群落分子多态性的丰富度,随着培养期的延长,施有稻秸的处理中土壤细菌群落多态性的变化远远复杂于空白对照土壤中的细菌群落变化;
Rice straw transformation takes place in soil but effects of rice straws on relations between soils and bacterial communities of the soils are still poorly understood. PCR and denaturing gradient gel electrophoresis (DGGE) were used to monitor development of bacterial communities in paddy soil and red soil, into which rice straw or rice straw plus biological agent was applied during incubating period, respectively. The total bacterial DNA from the two studied soils was extracted directly on D0,D5,D25,D45 and D70. The DNA from paddy soil was purified twice, but the DNA from red soil was done only once. The regions V3 of the 16S rDNA of bacteria from the two soils were amplified. DGGE profiles of the PCR productions were compared by similarity analysis. The results show simple DGGE profiles for controls of the two soils without rice straw. Rice straw was incubated in paddy soil and red soil for 70 days. DGGE profiles revealed that the composition of the bacterial community in soil with rice straw was more complex than CK during the incubating period. Meanwhile, the results show that peaks of the bacterial communities appeared at different times in the paddy soil and red soil, treated with rice straw during incubation times.
出处
《土壤学报》
CAS
CSCD
北大核心
2005年第2期270-277,共8页
Acta Pedologica Sinica
基金
上海市科技兴农重点攻关项目 (农科攻字 (2 0 0 0 ) 5 -6号 )资助
