摘要
用接合转移的遗传学方法证实了临床分离菌株吉戈菲肠杆菌(Enterobactergergoviae)3773含有一约60kb的可转移质粒,又用测耐药表型、酶水解率及基因片段杂交等方法证实了该质粒上有一编码产生超广谱β-内酰胺酶(ESbla)的基因.其大肠杆菌接合子除了头霉甲氧塞吩(cefoxitin)和亚胺硫霉素(imipenem)外,几乎对所有测定的β-内酰胺类药物都表现耐药,β-内酰胺酸抑制剂——棒酸(clavulanate)可抑制此ESbla的活性.携带此ESbla基因的质粒的一片段可与SHV-1的一结构基因片段杂交,说明此酶是SHV类的ESbla.
By conjugation experiment, an extended-spectrum β-lactamase (ESbla)encod-ed on a transferable plasmid of-60 Kilobases was found in a clinical strain of Enterobac-ter gergoviae isolated from Peking Union Medical College Hospital. Its E. coli transcon-jugant was resistant to all β-lactams tested except cefoxitin and imipenem. Clavulanic acid, a classical β-lactamase inhibitor,inhibited its activity. Hybridization with an intra-genic probe for SHV-1 revealed that this ESbla may be related to,or derived from SHV enzyme.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第2期106-112,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目
关键词
吉戈菲肠杆菌
SHV类
Β-内酰胺酶
Enterobacter gergoviae, SHV type extended-spectrum β-lactamase
