摘要
表达黄瓜花叶病毒(CMV)的卫星RNA的转基因烟草可以抗CMV的侵染.为了决定这些植物对其它病毒,如烟草花叶病毒(CMV)的安全性,我们对这些植物接种CMV.结果发现 卫星RNA可减弱TMV引起的症状,井降低病情指数,然而却对TMV在植物中的积累没有明显影响.这一发现有助于对卫星RNA抗病机制的理解,有助于含卫星RNA的生防制剂及表达病毒卫星RNA的转基因植物的应用.本文在国际上首次报道卫星RNA可减弱非相关病毒引起的症状.1 材料和方法1.1 病毒及植物烟草花叶病毒(TMV),烟草G140种子及枯班三生烟种子均为中国科学院微生物研究所病毒室保存;含黄瓜花叶病毒卫星RNA-R1基因的烟草G140(烟Sat-G140)为中国科学院微生物研究所病毒室培育,所用材料为第三代种子.
Transgenic tobacco plants that express satellite RNA of cucumber mosaic virus (CMV) are resistant to infection by CMV, To determine whether these plants also protect against other viruses, such as tobacco mosaic virus (TMV), they were inoculated with 0. 5μg/ml TMV. These plants showed much milder symptom by TMV and lower disease index than untransformed plants. The acumulation of TMV in these plants is similar to that in untransformed tobacco plants. These results demonstrate that transgenic tobacco plants expressing satellite RNA of CMV has a significant degree of protection against TMV, and benefit the understanding of the mechasim of virus satellite RNA-mediated resistance to virus and application of biological control agent containg virus satellite RNA and transgenic plants expressing virus satellite RNA. This is the first report that virus satellite RNA can reduce the symptom of unrelated virus.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第5期403-405,共3页
Acta Microbiologica Sinica
关键词
黄瓜花叶病毒
RNA
转基因烟草
病毒
Satellite RNA of CMV, TMV, Symptom reduction, Transgenic plant
