摘要
出现于北京协和医院的一耐头孢噻甲羧肟的临床分离菌株大肠杆菌(E.coli 5518),含有一约7.5kb的耐药质粒,编码产生一种新类型的超广谱β-内酰胺酶(ESbla).除了头霉甲氧噻吩和亚胺硫霉素外,产酶菌株对所测定的多种β-内酰胺类药物都耐药.该菌株耐β-内酰胺类药物的特性可接合传递给E.coli JP559菌株,与之一起转移的还有耐氨基糖甙类和磺胺类药物的特性.β-内酰胺酶抑制剂棒酸能抑制此酶的活性.此ESbla基因既不与SHV-1也不与TEM-1的结构基因片段杂交.
A novel extended-spectrum β-lactamase (ESbla) encoded on a plasmid of-7. 5kb, conferring resiztance to β-lactams tested except cefoxitin and imipenem, was found in a ceftazidime-resistant isolate of E. coli form our hospital. The resistance to β-lactams was transfered by conjugation to E. coli JP559 together with the the aminoglycosides and sulfonamide resistance. Clavulanate,one of β-lactamase inhibitors, inhibited its activity. This ESbla gene hybridized neither with an intra-genic fragment of SHV-1 nor with that of TEM-1. The molecular origin of that novel ESbla needed to be further studied.
出处
《微生物学报》
CAS
CSCD
北大核心
1994年第5期360-366,共7页
Acta Microbiologica Sinica
基金
国家自然科学基金资助项目
关键词
大肠杆菌
超广谱
β-内酶胺酶
E. coli,Extended-spectrum β-lactamase,Southern hybridization
