摘要
以榨菜子叶及带柄子叶等为外植体,在添加不同激素的MS培养基上,经不定芽诱导,不定芽伸长,生根等步骤,获得了完整的再生植株。结果表明:4d苗龄的子叶再生能力最强,带柄子叶的最佳苗龄为5～6d。子叶和带柄子叶分别在MS+BA2mg/L+NAA0.4mg/L+AgNO35mg/L和MS+BA2mg/L+NAA0.2mg/L+AgNO35mg/L的培养基中不定芽分化的效果较好。不同基因型间对子叶外植体的再生影响不大,但对带柄子叶的再生影响较明显。
A high efficiency regeneration system was established with the sessile cotyledon and cotyledon of tuber mustard (Brassica juncea Czern.et Coss.var.tumida) as experimental explants. Effects of hormone combinations, seedling age and cultivars on shoot and plant regeneration from different explants of tuber mustard were studied. Maximum shoot regeneration was obtained in MS + BA 2 mg·L^(-1) + NAA 0.4 mg·L^(-1) + AgNO_3 5 mg·L^(-1) and MS + BA 2 mg·L^(-1) + NAA 0.2 mg·L^(-1)+ AgNO_3 5 mg·L^(-1).Maximum root regeneration was obtained in MS + NAA 0.2 mg·L^(-1) + AgNO_3 5 mg·L^(-1).The sessile cotyledon from 4 days old seedlings had the highest regeneration ratio. But cotyledon from 5 days or 6 days old seedlings had the highest regeneration ratio. We also observed that effects of different genotypes on sessile cotyledon regeneration were inconspicuous, but that was obvious on cotyledon differentiation.
出处
《浙江农业学报》
CSCD
2005年第1期27-30,共4页
Acta Agriculturae Zhejiangensis
基金
浙江省重大科技项目资助(021102536)
关键词
榨菜
子叶
带柄子叶
离体再生
tuber mustard
sessile cotyledon
cotyledon
regeneration
in vitro
