摘要
目的 探索麻疹、风疹、流行性腮腺炎 (腮腺炎 )病毒感染的快速诊断方法。方法 利用多重逆转录 聚合酶链反应 (MRT PCR)方法对麻疹、风疹、腮腺炎病毒的相关基因检测进行研究。结果 直接从麻疹疫苗沪1 91 株 ,风疹疫苗BRDⅡ株 ,腮腺炎疫苗S79株 ,冻干麻疹、腮腺炎、风疹联合减毒活疫苗及临床麻疹、风疹、腮腺炎患者的标本中 ,同时检测出麻疹、风疹、腮腺炎病毒 6 3 5bp、4 1 1bp、5 1 9bp的特异性核酸片段 ,MRT PCR 1次扩增灵敏度均可分别达到 1TCID50 。结论 该方法能特异、敏感地检测临床标本中麻疹、风疹、腮腺炎病毒 ,是快速诊断麻疹、风疹。
Objective To discover a rapid MRT method for diagnosis of measles,rubella,and mumps infection. Methods MRT-PCR method was developed for detection of measles virus,rubella virus,and mumps virus. Results The measles vaccine strain(Shanghai-191),rubella vaccine strain(BRD Ⅱ),mumps vaccine strain(S_(79)),the live attenuated measles-rubella-mumps vaccines and the clinical samples collected from the measles,rubella,and mumps patients were analyzed.The amplified fragments of measles virus,rubella virus,and mumps virus in one reaction were 635bp,411bp,and 519bp respectively. Conclusion The sensitivity of this method to detect measles virus,rubella virus,and mumps virus was 1TCID_(50),1TCID_(50),and 1TCID_(50) respectively.We developed a specific,sensitive,and rapid MRT-PCR method for diagnosis of measles,rubella,and mumps infection.
出处
《中国计划免疫》
2005年第1期9-11,共3页
Chinese Journal of Vaccines and Immunization
