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结缔组织生长因子在增生性瘢痕成纤维细胞中的表达 被引量：1

Expression of connective tissue growth factor in fibroblasts from human hypertrophic scar
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摘要 目的 :探讨结缔组织生长因子 (CTGF)在人类增生性瘢痕成纤维细胞中的表达与瘢痕纤维化之间的关系。方法 :应用半定量逆转录聚合酶链反应 (RT- PCR)和 Western blot技术 ,检测了瘢痕组织原代培养的成纤维细胞中 CTGFm RNA及蛋白表达。结果 :CTGF m RNA及蛋白在人类增生性瘢痕成纤维细胞中高度表达 ,与正常皮肤组织的成纤维细胞比较 ,差异有显著性意义 (P<0 .0 1)。结论 :CTGF在人类增生性瘢痕成纤维细胞中呈高度表达 ,提示其在增生性瘢痕的纤维化进程中起着重要的作用 ,这为临床上寻找安全、有效的治疗增生性瘢痕药物提供了可能。 Objective:To investigate the relationship between connective tissue growth factor (CTGF) expression and fibrosis in human hypertrophic scar. Methods: Expression of CTGF mRNA and protein was detected in primarily cultured fibroblasts from human hypertrophic scar by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Results: Compared with normal skin, CTGF mRNA and protein were over-expressed in hypertrophic scar (P<0.01). Conclusion: It is suggested that CTGF overexpression play an important rale in the fibrosis of hypertrophic scar.
作者 吴平 何惠娟 张培华 张毅 江黎明
机构地区 广东医学院附属医院中心实验室 广东医学院整形外科研究所
出处 《广东医学院学报》 2005年第1期6-9,共4页 Journal of Guangdong Medical College
基金 广东省湛江市科技攻关项目 (编号 :湛科 [2 0 0 3] 68号医学五 - 4 )
关键词 增生性瘢痕 成纤维细胞 结缔组织生长因子 逆转录-聚合酶链反应 蛋白印迹 hypertrophic scar fibroblast connective tissue growth factor RT-PCR Western blot
分类号 R965 [医药卫生—药理学]
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参考文献12

  • 1刘剑毅,李世荣,纪淑兴.病理性瘢痕中结缔组织生长因子基因的表达[J].中国修复重建外科杂志,2003,17(6):436-438. 被引量：28
  • 2Border WA,Noble NA.Transforming growth factor beta in tissue fibrosis[J].N EngI J Med,1994,331(19):1286-1292.
  • 3Leask A,Holmes A,Abraham DJ.Connective tissue growth factor: a new and important player in the pathogenesis of fibrosis[J].Curr Rheumatol Rep,2002,4(2):136-142.
  • 4Bradham DM,Igarashi A,Potter RL,et al.Connective tissue growth factor: a cystein-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gone product CEF-10[J].J Cell Biol,1991,114(6):1285-1294.
  • 5Gupta S,Clarkson MR,Duggan J,et al.Connective tissue growth factor: potential role in glomerulosclersis and tubulointerstial fibrosis.Kidney Int,2000,58(4):1389-1399.
  • 6Solomon A,Li DQ,Lee SB,et al.Regulation of collagenase,stromelysin,and urokinase-type plasminogen activator in primary pterygium body fibroblasts by inflammatory cytokines[J].Invest Ophthalmol Vis Sci,2000,41(8): 2154-2163.
  • 7Kucich U,Rosenbloom JC,Herrick DJ,et al.Signaling events required for transforming growth factor-β stimulation of connective tissue growth factor expression by cultured human lung fibroblasts[J].Arch Bioch Bioph,2001,395(1):103-112.
  • 8Fan WH,Karnovsky MJ.Increased MMP-2 expression in connective tissue growth factor over-expression vascular smooth muscle cells[J].J Biol Chem,2002,27(12):9800-9805.
  • 9Igarashi A,Nashiro K,Kikuchi K,et a1.Connective tissue growth factor gene expression in tissue sections from localized scleroderma,keloid,and other fibrotic skin disorders.J Invest Dematol,1996,106(4):729-733.
  • 10Igarashi A,Nashiro K,Kikuchi K,et al,Significant correlation between connective tissue growth factor gene expression and skin sclerosis in tissue sections from patients with systemic sclerosis[J].J Invest Dermatol,1995,105(2):280-284.

二级参考文献15

  • 1Leask A, Holmes A, Abraham DJ. Connective tissue growth factor: a new and important player in the pathogenesis of fibrosis. Curr Rheumatol Rep, 2002; 4(2): 136.
  • 2Letterio JJ, Bottinger EP. TGF-beta knockout and dominant-negative receptor transgenic mice. Miner Electrolyte Metab, 1998; 24(2-3) : 161.
  • 3Bradham DM, Igarashi A, Potter RL, et al. Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10. J Cell Biol, 1991, 114(6): 285.
  • 4Grotendorst GR, Okochi H, Hayashi N. A novel transforming growth factor beta response element controls the expression of the connective tissue growth factor gene. Cell Growth Differ, 1996; 7(4): 469.
  • 5Holmes A, Abraham DJ, Sa S, etal. CTGF and SMADs, maintenance of scleroderma phenotype is independent of SMAD signaling. J Biol Chem, 2001, 276(14) : 10 594.
  • 6Border WA, Noble NA. Transforming growth factor beta in tissue fibrosis. N Engl J Med, 1994, 331: 1286-1292.
  • 7Takehara K. Growth regulation of skin fibroblasts. J Dermatol Sci,2000, 24(Suppl 1 ): S70-77.
  • 8Igarashi A, Nashiro K, Kikuchi K, et al. Connective tissue growth factor gene expression in tissue sections from localized scleroderma,keloid, and other fibrotic skin disorders. J Invest Dermatol, 1996,106: 729-733.
  • 9Darzi MA, Chowdri NA, Kaul SK, et al. Evaluation of various methods of treating keloids and hypertrophic scars: a 10-year follow-up study. Br J Plast Surg, 1992, 45: 374-379.
  • 10Yamamoto S, Kitadai Y, Tsuchida A, et al. Expression of plateletderived endothelial cell growth factor/thymidine phosphorylase in human gallbladder lesions. Eur J Cancer, 2000, 36: 257-263.

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广东医学院学报
2005年 第1期

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