摘要
本文通过鸡胚接种的方法就蜂胶乙醇浸出物对禽流感H9亚型Shandong/chick-en/98株的灭活作用进行了研究。结果表明,常温下3分钟、30分钟,37℃3分钟、30分钟,含蜂胶乙醇浸出物0.1mg/mL的95%乙醇稀释液,含蜂胶乙醇浸出物2.5mg/mL的47.5%乙醇稀释液,含蜂胶乙醇浸出物10mg/mL的生理盐水稀释液,接种鸡胚后病毒HA测定为0。这充分说明以上浓度蜂胶乙醇浸出物对禽流感H9亚型Shandong/chicken/98株具有完全的灭活作用。37℃3分钟、30分钟,含蜂胶乙醇浸出物0.1mg/mL的47.5%乙醇稀释液,含蜂胶乙醇浸出物6.25mg/mL的生理盐水稀释液对禽流感H9株有部分灭活作用。
The inactivation effect of ethanol extract of propolis on the H9 subtype Avian Influenza Virus(AIV) Shandong/chicken/98 Strain was observed through chicken embryo inoculation. The result demonstrated that,H9 subtype AIV Shandong/chicken/98 Strain can be completely inactivated at both room temperature and 37℃ in 3 minutes by 95% ethanol dilution,47.5% ethanol dilution and physiological saline,which containing 0.1mg,0.1mg and 10mg ethanol extract of propolis per ml,respectively;and can be inactivated at room temperature in 3 minutes by 47.5% ethanol dilution containing 1mg ethanol extract of propolis per ml as well.
出处
《中国家禽》
北大核心
2005年第3期12-14,共3页
China Poultry
