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植物络合素的测定方法 被引量:5

Analysis methods of phytochelatins
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摘要 植物络合素是一类富含巯基 ,并能够与重金属离子相结合的多肽。植物络合素具有共同的结构 :[γ- Glu- Cys]nGly (n =2~ 11)。植物络合素是植物重金属抗性的要素之一。植物络合素的测定是一种植物重金属抗性机理的研究技术。介绍了植物络合素测定的理论 ,并综述了现有的植物络合素测定的方法 :分光比色法、凝胶过滤法、液相色谱法和毛细管电泳法。其中 ,着重阐述了液相色谱法在植物络合素测定中的应用。 Phytochelatins is a kind of peptides rich in cysteine,which can bind the ion of heavy metals.Phytochelatins has a common structure: [γ-Glu-Cys ] n Gly (n=2~11),and it is one of the key factors of plant heavy metal tolerance mechanism.Its analysis methods can be applied to study plant heavy metal tolerance mechanism.The article introduced the basic theory of phytochelatins analysis,and analysis methods including Spectrophotometer,HPLC and CE,among which the HPLC was presented in detail.
出处 《生态学杂志》 CAS CSCD 北大核心 2005年第3期330-334,共5页 Chinese Journal of Ecology
基金 国家重点基础研究发展规划项目(2002CB410808) 国家自然科学基金资助项目(40225002)
关键词 植物络合素 测定 液相色谱 phytochelatins,analysis methods,HPLC
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  • 1高华,张玉秀,柴团耀.植物络合素和植物络合素合酶的研究[J].西北植物学报,2001,21(4):779-790. 被引量:8
  • 2Aher BA, Price NM, Morel FMM. 1994. Phytochelatin production by marine phytoplankton at low free metal ion concentrations: Laboratory studies and field data from Massachusetts Bay[J]. PNAS, 91:8433~8436.
  • 3Ebbs S, Lau I, Ahner B, et al. 2002. Phytochelatin synthesis is not responsible for Cd tolerance in the Zn/Cd hyperaccumulator Thlaspi caerulescens (J. & C. Presl) [J]. Planta, 214:635~640.
  • 4Hart JJ, Wrlch RM, Norvell WA, et al. 2002. Measurement of thiol-containing amino acids and phytochelatin (PC2) via capillary electrophoresis with laser-induced fluorescence detection[J].Electrophoresis, 23: 81 ~ 87.
  • 5Hartley-Whitaker J, Ainsworth G, Vooijs R, et al.2001. Phytochelatins are involved in differential arsenate tolerance in Holcus lanatus [ J ].Plant Phys., 126:299~306.
  • 6Hiroki K, Kyoko S, Takashi Y, et al. 1998. Separation of respective species of phytochelatins and their desglycyl peptides (class Ⅲ metallothioneins) and the precursors glutathione and g-glutamylcysteine with capillary zone electrophoresis [ J ]. J. Chromatography A, 803: 315 ~ 320.
  • 7Newton GL, Dorian R, Fahey RC. 1981. Analysis o( ) biological thiols: Derivatization with monobromobimane by reverse-phase high-performance liquid chromatography [ J ]. Anal. Biochem.,114: 383 ~ 387.
  • 8Schmoger MEV, Oven M, Grill E. 2000. Detoxification of arsenic by phytochelatins in plants[J ]. Plant Phys., 122: 793 ~ 801.
  • 9Senft AP, Dalton TP, Shertzer HG. 2000. Determining glutathione and glutathione disulfide using the fluorescence probe o-phthalaldehyde[J]. Anal. Biochem., 280: 80~ 86.
  • 10Sheller FEC, van Heerwaarden LM, Koevoets PLM, et al. 2000.Derivatization of phytochelatins from Silene vulgaris, induced upon exposure to arsenate and cadmim: Comparison of derivatization with ellman's reagent and monobromobimane[J]. J. Agric. Food Chem., 48:4014~4019.

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