摘要
目的 研究化疗药环磷酰胺对癌细胞的细胞周期、DI值、PI值等某些生物学特性的影响及其诱导癌细胞凋亡的作用。方法 用BALB/c小鼠 ,荷腹水性肝癌 ,经腹腔给药CTX后 ,抽取腹水癌细胞进行形态学观察和流式细胞光度分析研究。结果 在环磷酰胺的作用下 ,癌细胞发生了凋亡 ,形态上观察到了许多凋亡细胞和凋亡小体 ;流式细胞光度分析发现 ,药物作用后 ,细胞周期发生了巨大变化 ,大多数癌细胞被阻断在G2M期 ,而G1期癌细胞明显减少 (P <0 0 5～P <0 0 1) ;同时 ,给药组癌细胞的DNA值和PI值亦明显增高 (P <0 0 5～P <0 0 1)。结论 环磷酰胺能够诱导癌细胞凋亡 ,而且对癌细胞的细胞周期一般规律有较大的干扰。分析认为这对癌症临床治疗会有一定启示。
Objective To study the effects of cyclophosphamide on some biological characteristics of l iver carcinoma cells. Method BALB/c mice were injected peritoneally . At 10-{th} day after treatment, the ascites cancer cells were taken, the mo r phologyic and flow cytometric analysis were carried out. Results We found that in cyclophosphamide group, many apoptotic bodies were observed and t he cancer cells in G2M phases were blocked, but the cancer cells in G1 phase dec reased significantly (P<0^05～P<0^01). Meanwhile, DNA index (DI) and proli feration index (PI) increased significantly (P<0.05～P<0.01). Conclu sion Cyclophosphamxide could induce Apoptosis of cancer cells; and also i nterfere the cell cycle of the cancer cells, which may be of value in the cancer treatment.=
出处
《实验动物科学与管理》
2005年第1期12-14,共3页
Laboratory Animal Science & Administration
关键词
环磷酰胺
癌细胞凋亡
细胞周期
Cyclophosphamide
Appotosis of cancer cells
Cell c ycle
