摘要
以黑籽南瓜为砧木,以津绿21-1和津研4号2个黄瓜品种为接穗,对嫁接植株的光合指标及叶片激素含量和可溶性蛋白进行对比研究.结果表明,2个品种嫁接植株的净光合速率(Pn)、气孔导度(Gs)和胞间CO2浓度(Ci)均极显著高于自根植株;嫁接植株叶片GA和ABA含量也与自根植株存在显著差异,嫁接植株叶片ABA/GA值在测定的各个时期均显著高于自根植株;通过等电聚焦聚丙烯酰胺双向电泳(IEF-SDS PAGE)对叶片可溶性蛋白进行分析对比,发现在嫁接植株叶片中产生了1～2种特异蛋白.
Using black seed pumpkin(Cucurbita ficifolia Bouché)as root stock,and two cucumber(Cucumis sativus L.)cultivars Jinlü 21-1 and Jinyan 4 as scions,grafting was made to compare the differences in photosynthetic parameters,leaf GA and ABA contents,and leaf soluble protein components between grafted and self-root plants.The results showed that photosynthetic rate,stomatal conductance and intercellular CO 2 concentration of grafted plants were significantly higher than those of self-root plants.Leaf GA and ABA contents at different stages of grafted plants were significantly different to those of self-root plants.ABA/GA values of leaves of grafted plants were significantly higher than those of self-root plants at various measuring stages.Soluble proteins extracted from the leaves of grafted and self-root cucumber plants were analyzed using the technique of two dimensional polyacrylamide gel electrophoresis.The results indicated that there were one to two additional protein spots from grafted cucumber.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2005年第1期16-19,共4页
Journal of Nanjing Agricultural University
基金
教育部留学回国人员启动基金资助项目 ( [2001] 498)
江苏省科技厅科技成果示范推广计划资助项目 (BC2003306 )
江苏省科技厅农业科技攻关计划资助项目 (BE2002304)
关键词
黄瓜
嫁接
光合参数
激素
蛋白质
cucumber
graft
photosynthetic parameter
hormone
protein
