摘要
通过根癌农杆菌介导花期喷雾,将内切几丁质酶基因导入拟南芥哥伦比亚生态型的野生型植株中,转化的种子经潮霉素抗性筛选,获得抗性幼苗11棵,转化频率为0.22%,经叶片PCR或基因组DNAPCR,扩增出外源基因ThEn42的特异性DNA片断,当代植株表现出不育、矮化和生长发育正常等不同表型。接种油菜菌核病菌(Sclerotiniasclerotiorum)后,T1代植株对病菌抗性显著提高。
Arabidopsis thaliana (Col0) was transformed by spraying Agrobacterium tumuficience with Trichoderma endochitinase gene ThEn42 at initial flower stage. Eleven seedlings (transformed at about 0.22 percentage) exhibited resistance to hygromycin, the marker for the screening. The DNA fragment unique to endochitinase ThEn42 as amplified by Arabidopsis leaf-PCR or genomic DNA PCR. Unfertile, dwarf and normal phenotypes appeared in the T1 generation of plants. In addition, their resistance to S. sclerotiorum,an important pathogen on canola, was obviously increased.
出处
《上海交通大学学报（农业科学版）》
2005年第1期36-40,45,共6页
Journal of Shanghai Jiaotong University(Agricultural Science)
基金
上海市科技兴农重点攻关项目资助(沪农科攻字2003第3-4号)
