摘要
以有效的热溶水提法,从菱叶红景天植物中得到粗多糖RHP。RHP经乙醇分级得三个级分RHP-I,RHP-II,RHPIII,取RHPIII脱蛋白,脱色,DEAD-纤维素柱层析,Saphadex G-200柱层析分离纯化,色谱鉴定及初步理化特性分析,得一纯度较高的均一多糖RHPS。PC分析其单糖组成有L-阿拉伯糖、L-鼠李糖、D-葡萄糖和另一个待确定的组分组成。
The R. Henryi Polysaccharides (RHP) were isolated from R. henryi by a high efficient water-extrating method. RHP- I,RHP-II and RHP-III were obtained by ethanol precipitating in RHP. After using RHP-III, eliminating albumen, decolorating, dialysis and freeze drying, it was purified by DEAE-cellulose column chromatography. As purified polysaccharides, RHPs were obtained and identified by Saphadex G-200 column chromatography and UV and IR spectrum.The results analysed by paper chromatography showed that its monnosaccharides were arahinose(Ara), rhamnose (Rha) and glucose(Glc), but a monnosaccharide should be further identified.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第3期60-63,共4页
Food Science
关键词
菱叶红景天多糖
提取
纯化
鉴定
理化特性
R.Henryi polysaccharides(RHP)
extraction
purification
identification
physical and chemical charac- teristics
