摘要
本文对桑叶多糖的提取、分离纯化和单糖的组成进行了研究。桑叶经热水提取乙醇沉淀得多糖粗品,经脱蛋白,乙醇分级沉淀、DEAE-纤维素柱和Sephadex G-100柱层析,纯化得MP11、MP12、Mp13三个多糖组分。经糖腈乙酰化处理后进行气相色谱分析得知MP11由Rha、Ara、Xyl、Man、Glu、Gal组成,其比例为21:16:3:3:1:20;MP12由Rha和Glu组成,其比例为3:1;MP13主要由Rha组成。为桑叶多糖的进一步研究提供了基础。
The crude polysaccharides from Leaves of Morus alba L. were obtained by extracting hot water and precipitating with alcohol. The free proteins were removed from crude polysaccharides and then the crude Morus polysaccharides (MP1) were Isolated and purified by DEAE-cellulose column and Sephadex G-100 gel column chromatography. The result showed that MP1 was composed of three pure polysaccharides (MP11,MP12,Mp13). After converting the hydrolyzed polysaccha- ride into acetylated aldononitriles it was analysis by gas chromatography. The chemical components and the ratio showed follows:MP11:Rha:Ara:Xyl:Man:Glu:Gal=21:16:3:3:1:20;MP12:Rha:Glu=3:1;MP13's component was mainly Rha. All these work could establish foundation for the further study of Morus Polysaccharides.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第3期181-184,共4页
Food Science
基金
江苏省农业科技攻关项目(BE2004352)
关键词
桑叶
多糖
分离纯化
气相色谱
组成
leaves of Morus alba L.
polysaccharides
isolation purification
gaschromatography
component
