摘要
本实验应用聚丙烯酰胺凝胶盘状电泳方法对莫桑比克罗非鱼(Tilapia mossambica)和尼罗罗非鱼(Tilapia milotica)及其杂交种——福寿鱼(T.missambica ?×T.nilotica ?)的血清蛋白进行了分离,同时对其雌性特异蛋白进行了氨基酸组成分析。莫桑比克罗非鱼的血清蛋白电泳图型具有 12—14条蛋白质带,尼罗罗非鱼有12—13条蛋白质带,杂交种—福寿鱼有12—15条蛋白质带;三种鱼血清蛋白电泳图型在雌、雄性别上有明显的差异。莫桑比克罗非鱼和尼罗罗非鱼的成熟雌鱼有两条新的蛋白质带,这些带都比同种的雄鱼所显示的颜色深。杂交种——福寿鱼的血清蛋白电泳图型,为其亲本的中介型。 此外,本文还对雌性特异蛋白的蛋白质带,进行了氨基酸组成分析。
Serum proteins of Tilapia mossambica. Tilapia nilotica and their hybrid (femaleT. Mossambica×male T. nilotica) were examined by using 7% polyacrylamide gelelectrophoresis.The T. mossambica serum was isolated into 12--14 protein bands, T. niloticaserum into 12--13 protein bands and the bybrid serum into 13--15 protein bands.Sexual differences in the electrophoretic patterns of serum proteins of the twospecies and their hybrids were compared. The electrophoretic patterns of bandingshowed two new bands in the serum of mature female T. mossombica. They were notobserved in the males. But in the females of T. mossambica and of T. nilotica theprotein bands were broder and darker in colour than those of the males. The eletropho-retic pattern of the hybrids was transient among several components.Various staining methods were used: (a) for total proteins with amide black dyetwo new protein bands appeared; (b) for glycoproteins with the periodic acid-Schiffreaction, the two new protein bands were not visible. However, other protein bandsshowed red colour;(c) for lipoprotein with sudan black B there were two markedprotein bands.For scanning electropherograms on serum of the fish and amins acid compositionof female specific serum protein,ultra-violet-spectro photometer was used. This femalespecific serum protein was characterized by relatively high contents of glycine, pheny-lalanine and amide NH. but the absence of histidine and tyrosine,
出处
《水产学报》
CAS
1985年第3期265-273,共9页
Journal of Fisheries of China
