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大蒜蒜瓣离体繁殖研究 被引量:9

IN VITRO PROPAGATION OF GARLIC CLOVES
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摘要 以山东苍山大蒜蒜瓣为外植体进行离体培养,在MS+KT_(1.0)+NAA_(0.005)(单位:mg/l,下同)培养基上,外植体可直接分化出新梢。新梢在MS+KT_(1.0)+IBA_(1.0)+NAA_(2.0)培养基上进行2次继代增殖,然后在B_5+BA_(1.0)培养基上进行1次继代增殖,繁殖系数依次为7.6,6.5,4.0。共经160~180天,单瓣蒜累积繁殖系数可达1640。新梢在MS+NAA_(0.1)+BA_(0.1)培养基上生根率可达87.5%。在MS+NAA_(2.0)+BA_(0.05)培养基上,小鳞茎发生率可达85.7%。采用有根单鳞茎试管苗移栽,成活率为92%。细胞学观察表明,该途径保持了原材料的遗传稳定性。 In vitro propagation of garlic cloves was discussed in this paper.Proliferated shoots were produced directly when the garlic cloves pieces of Cangshan were cultured in vitro on the medium MS suplemented with 1.0 mg/L KT and 0.005 mg/L NAA. When the shoots were subcultured twice on the medium MS suplemented with 1.0 mg/L KT,1.0 mg/L IBA and 2.0mg/L NAA,shoots could be proliferated more,Propagation coeffcient was 7.6 and 6.5.Then subcultured on the medium B_5 suplemented with BA 1.0 mg/L,propagation coef-ficient was 4.0.Through the initial culture and three times subculture during 160~180 days,total shoots propagated from one clove were 1640.When the shoots were cultured on the medium MS suplemented with 0.1mg/L BA and 0.1mg/L NAA,rootability got to 87. 5%. Bulblets could be formated on the medium MS suplemented with 2.0mg/L NAA and 0.05 mg/L BA. When plantlets with roots and bulblets were transplanted,the survival rate got to 92%. Cytogenetic analysis on the tube plantlets from different generation showed that there were 16 chromosomes in all single cells observed,no variation was found,So the ge-netic type of primary material was maintained well.
出处 《西北农业学报》 CSCD 1994年第3期62-66,共5页 Acta Agriculturae Boreali-occidentalis Sinica
基金 国家自然科学基金
关键词 大蒜 蒜瓣 繁殖 离体培养 Garlic Cloves In vitro propagation Bulblets
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二级参考文献5

  • 1张忠新,植物生理学通讯,1985年,6期,41页
  • 2张忠新,实验生物学报,1983年,16卷,3期,241页
  • 3陈祖铿,1976维管植物实验胚胎发生,1983年
  • 4谢浩,植物学报,1981年,3卷,11期,57页
  • 5La Y T,Korean J Pl Prot,1973年,12卷,93页

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