摘要
在干冰环境下粉碎的决明子,用甲醇和氯仿提取脂类,通过TLC来分离脂类中的组成成分;并将分离后的各组分脂肪甲基化,用100m×0.25mm的GC毛细管柱来测定脂类中脂肪酸的组成。结果表明,决明子脂类由TG、CE、FFA等组成,且TG为主要成分占74.689%,FFA和CE分别为17.840%和7.470%,这种差异主要是由于16:0、18:0、18:1C、9c12c18:2和18:3n3含量不同引起。CE中饱和脂肪酸比TG和FFA高,分别为30.8315%、26.469%和24.221%,除16:0脂肪酸的含量FFA和TG比CE高(分别为17.613%、16.287%和12.148%)外,CE中14:0、20:0、21:0、22:0和24:0脂肪酸都比FFA和TG中相应的脂肪酸高得多。多不饱和脂肪酸为主要的脂肪酸,且其在FFA和TG中比CE高得多,分别为46.156%、45.107%和41.042%,这种差异主要是9c12c18:2含量(分别为44.252%、43.598%和38.833%)不同所致。总之,决明子脂类中FFA和TG组成成分极为相近,而与CE的组成有较大的差异。
The lipids were extracted by using methanol and chloroform after comminuting the seeds of Cassia Tora in the environment of dry ice. Different components of lipids separated with thin layer chromatogram (TLC) were methylated and determined by GC equipped with 100m×0.25mm capillary column. The results indicated that the lipids were composed of triglyceride (TG), cholesterol ester (CE) and free fatty acids (FFA). Among them TG, a main component, was 74.689%, while FFA and CE were 17.840% and 7.470% respectively. This difference was resulted from 16:0, 18:0, 18:1C, 9c12c18:2 and 18: 3n3. Saturated fatty acids (SFA) in CE was higher than that in TG and FFA, 30.8315%,26.469% and 24.221% respectively, in which 14:0, 20:0, 21:0,22:0 and 24:0 in CE were all higher than that in TG and FFA except for 16:0 (12.148%,16.287% and 17. 613% respectively). Polyunsaturated fatty acids (PUFA) had the highest concentration in TG, CE and FFA, and were higher in FFA and TG than in CE, 46.156%, 45.107% and 41.042% respectively. This difference was mainly caused by different content of 9c12c18:2 (44.252%,43.598% and 38.833% in FFA, TG and CE respectively). In conclusion, lipids in FFA were similar to those in TG, but differed from those in CE.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2005年第2期162-165,共4页
Food Science
基金
国家留学基金委项目
江苏省自然科学基金(0220013)
江苏省卫生厅2003年中药基础研究基金
关键词
决明子
脂类
提取
测定
Cassia Tora
lipids
extraction
determination
