摘要
香荚兰根腐病是影响香荚兰生产的一个制约因素,其病原菌为尖孢镰刀菌和茄类镰刀菌。试验应用同工酶电泳技术对引起香荚兰根腐病致病性不同的12个菌株(其中尖镰孢9株,茄镰孢3株)进行酯酶同工酶谱分析。结果发现酯酶同工酶的活性区主要集中在中间带区和快带区。对12个菌株的酯酶同工酶带的Rf值进行UPGMA聚类分析,发现在相似性0 71的水平上,能将尖孢镰刀菌和茄类镰刀菌区分;在相似性0 86的水平上,能将用传统的致病性鉴定方法鉴定出的强、中、弱致病菌株及非致病菌株分开。试验的目的在于寻找与致病性/非致病性相关的同工酶标记。
Vanilla root rot has been a limiting factor to vanilla production in most regions in which it is grown. The causal reagents of vanilla root rot were Fusarium oxysporum f.sp. vanillae and Fusarium solani. The technique of isozyme electrophoresis was applied to analyze 12 of causal reagents of vanilla root rot, including 9 isolates of F.oxysporum and 3 isolates of F.solani. The aims of this research was to find the marker related with pathotypes. The EST (esterase) electrophoresis analysis showed: EST was almost distributed in middle and fast areas. All the EST bands were clustered by Unweighted Pair Group with Mathematic Average (UPGMA). The dendrogram of UPGMA showed that F.oxysporum f.sp.vanillae and F.solani can be differentiated based on 0.71 analogical level; different pathotypes can be differentiated based on 0.86 analogical level.
出处
《云南农业大学学报》
CAS
CSCD
2005年第1期27-30,共4页
Journal of Yunnan Agricultural University
基金
云南省科技攻关项目(2000A3-01)
关键词
香荚兰
尖镰孢
茄镰孢
酯酶同工酶
Vanilla root rot
Fusarium oxysporum
Fusarium solani
Esterase
