摘要
目的建立高效液相色谱测定四制香附丸中橙皮苷含量的方法.方法以十八烷基硅烷键合硅胶为填充剂;乙腈-0.2%磷酸水溶液(21:79)为流动相;检测波长为284 nm.结果线性关系为Y=1.9848 X+2.1951,相关系数r=0.9995,线性范围在70.24～561.92 ng,平均回收率101.34%(n=6),RSD=1.08%.结论本法操作简便准确,可作为四制香附丸中橙皮苷含量的质量控制方法.
Aim To establish an HPLC determination method of hesperidin in sizhixingfu pill.Methods The analyti calcolumn was C 18 .The mobile phase was acetonitrile: 0.2%-phosphoric acid (21 ∶79) at the detetection wavelength of 284 nm.Result Linearity range was 70.24~561.92 ng(r=0.995),Y=1.9848 X+2.1951;The average recovery was 101.34%(n=6),RSD=1.08%. Conclusion The method can be used in the quality control of sizhixiangfu pill.
出处
《安徽医药》
CAS
2005年第3期206-207,共2页
Anhui Medical and Pharmaceutical Journal
