摘要
目的:探讨白细胞介素-1β(interleukin-1β,IL-1β)对急性分离的大鼠脊髓背角感觉神经元电生理的影响及其作用机理。方法:酶解分离获得单个脊髓背角感觉神经细胞,采用标准全细胞膜片钳技术记录用药前后神经元的电压门控钠离子通道电流(INa)、钙电流(ICa)和钾电流(IKv)的变化。结果:IL-1β抑制内向INa和ICa,增强外向IKv,IL-1β的作用随浓度升高而增强;IL-1β改变各离子电流的I-V曲线幅度,而对曲线形态无明显影响;IL-1受体拮抗剂(IL-1Ra)对IL-1β的电生理作用无显著影响。结论:IL-1βI对大鼠脊髓背角感觉神经元电压门控离子通道有调控作用,L-1受体非依赖性的电生理作用可能参与IL-1β的感觉神经保护作用。
Objective:To observe electrophysiological effect of interleukin-1β(IL-1β) on voltage-dependent ion channels of sensory neurons in spinal dorsal horn and its preliminary mechanism.Method:Single sensory neurons of the dorsal horn in the spinal cord of SD rat were collected by enzymaolysis.The whole-cell patch clamp recording technique was used to record the amplitude changes of voltage-dependent sodium current(INa),calcium current(ICa) and potassium current(IKv) with IL-1β and the impact of IL-1 receptor antagonist (IL-1Ra) on those changes.Result:3pg/ml,300pg/ml IL-1β reduced the amplitudes of INa by (34.5±4.6)% and (52.8±7.5)%,and reduced the amplitudes of ICa by (24.6±3.2)% and (76.3±9.6)%,and increased amplitude of IKv by(42.5±6.2)% and(110.7±15.9)%,respectively.IL-1β changed the amplitude of I-V curves of ion currents,but did not affect their shapes significantly.IL-1Ra did not impact the electrophysiological effect of IL-1β.Conclusion:IL-1β involves in the neuroprotection of spinal cord neurons by IL-1 receptor-independent pathway.
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2005年第2期112-116,共5页
Chinese Journal of Spine and Spinal Cord
