摘要
目的从基因和蛋白水平探讨中药成分苦参碱 (matrine)对大肠癌HT-2 9细胞环氧化酶-2(COX-2 )表达的影响。方法分别采用RT-PCR、Western blot、ELISA等方法检测不同浓度苦参碱处理HT-2 9细胞前后COX-2mRNA、蛋白及其产物前列腺素E2 (PGE2 )水平的改变。结果苦参碱可抑制HT-2 9细胞COX-2mRNA、蛋白表达及PGE2 合成水平 ,但对COX 1无影响。当苦参碱 2 .0mg/ml处理时 ,COX 2mRNA表达在处理后 6h和 9h时抑制率均为 10 0 % ;细胞培养液PGE2 合成水平在 9h时抑制率为6 3.9% ;COX-2蛋白表达在 12h和 2 4h时抑制率分别为 4 8%和 10 0 %。结论苦参碱具有选择抑制HT-2 9细胞COX-2的基因转录、蛋白表达和功能活性等作用 ,在一定浓度和时间范围内 。
explore the effect of matrine on cyclooxygenase-2 (COX-2) expression in colon cancer HT-29 cell line at the level of gene and protein.Methods Levels of mRNA and protein expression of COX-2, and its synthesized product prostaglandin E 2 (PGE 2) of colon cancer HT-29 cell line were detected by RT-PCR, Western-blot, ELISA respectively before and after treatment of matrine in different concentrations. ResultsMatrine had inhibitory effect on the mRNA and protein expression of COX-2, and synthesis of PGE 2 in colon cancer HT-29 cell line, but had no effect on COX-1. When HT-29 cell line was treated with 2.0 mg/ml of matrine, the inhibitory rate on COX-2 mRNA expression were 100% at 6 hrs and 9 hrs after treatment; the inhibitory rate on PGE 2 synthesis was 63.8% at 9 hrs after treatment; and that on COX-2 protein expression was 48% and 100% 12 hrs and 24 hrs after treatment, respectively. ConclusionMatrine has selective inhibitory effect on gene transcription, protein expression and functional activity of COX-2 in HT-29 cell line, which is time-dependent and concentration-dependent within certain range of concentration and acting time.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2005年第3期240-243,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
浙江省自然科学基金项目青年人才专项基金 (No.8C0 1 0 55)
浙江省中医药管理局项目 (No .2 0 0 3C0 94)
关键词
苦参碱
大肠癌
HT-29细胞
环氧化酶-2
表达
中药
中医药疗法
matrine
colon cancer HT-29 cell line
cyclooxygenase-2
protein expression
gene transcription
prostaglandin E 2
